ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9589B
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Biotin
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu5
Product Specifications
Immunogen
Human ErbB2/Her2
Specificity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.
MCF-7 human cell line was stained with Biotinylated Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9589B, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
ErbB2/Her2 in SK-BR-3 Human Cell Line.
ErbB2/Her2 was detected in both live and fixed SK-BR-3 human breast cancer cell line and but not detected in fixed U937 human histiocytic lymphoma cell line. SK-BR-3 live cells were incubated for 1 hour at 4°C with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted at 5 µg/mL with HBSS. SK-BR-3 and U937 fixed cells were incubated for 2 hours at room temperature with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted with antibody diluent. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MCF-7 Human cell line
Sample: MCF-7 Human cell line
Immunocytochemistry
5-25 µg/mL
Sample: Live and fixed SK-BR-3 human breast cancer cell line
Sample: Live and fixed SK-BR-3 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Specific Notices
For research use only
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