ERK3/MAPK6 Antibody (OTI5E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47854

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5E1

Concentration

1 mg/ml

View all ERK3 Antibodies »

Product Summary for ERK3/MAPK6 Antibody (OTI5E1)

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens mitogen-activated protein kinase 6 (MAPK6).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK3/MAPK6 Antibody (OTI5E1)

Western Blot: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Western Blot: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.
Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (OTI5E1) [NBP1-47854]

Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.

Applications for ERK3/MAPK6 Antibody (OTI5E1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK3

ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Entrez Gene IDs

5597 (Human)

Gene Symbol

MAPK6

Additional ERK3 Products

Product Documents for ERK3/MAPK6 Antibody (OTI5E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK3/MAPK6 Antibody (OTI5E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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