ERK5/BMK1 Antibody (OTI3H2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03371
![Western Blot: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]](https://resources.bio-techne.com/images/products/ERK5-Antibody-3H2-Western-Blot-NBP2-03371-img0002.jpg "Western Blot: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3H2
Concentration
0.6 mg/ml
Product Summary for ERK5/BMK1 Antibody (OTI3H2)
Immunogen
Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
88.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERK5/BMK1 Antibody (OTI3H2)
Western Blot: ERK5 Antibody (3H2) [NBP2-03371] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK5 monoclonal antibody.
![Immunocytochemistry/ Immunofluorescence: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]](https://resources.bio-techne.com/images/products/ERK5-Antibody-3H2-Immunocytochemistry-Immunofluorescence-NBP2-03371-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]")
Immunocytochemistry/ Immunofluorescence: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]
Immunocytochemistry/Immunofluorescence: ERK5 Antibody (3H2) [NBP2-03371] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERK5.![Flow Cytometry: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]](https://resources.bio-techne.com/images/products/ERK5-Antibody-3H2-Flow-Cytometry-NBP2-03371-img0001.jpg "Flow Cytometry: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]")
Flow Cytometry: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]
Flow Cytometry: ERK5 Antibody (3H2) [NBP2-03371] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERK5 antibody, and then analyzed by flow cytometry.Applications for ERK5/BMK1 Antibody (OTI3H2)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ERK5/BMK1
Long Name
Extracellular Signal-regulated Kinase 5
Alternate Names
BMK1, MAPK7
Entrez Gene IDs
5598 (Human)
Gene Symbol
MAPK7
Additional ERK5/BMK1 Products
Product Documents for ERK5/BMK1 Antibody (OTI3H2)
Product Specific Notices for ERK5/BMK1 Antibody (OTI3H2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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