FAK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84750
Key Product Details
Species Reactivity
Validated:
Human, Porcine
Cited:
Porcine
Predicted:
Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRE
Reactivity Notes
Porcine reactivity reported in the scientific literature (PMID: 30257480).
Marker
Focal Adhesion Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAK Antibody
Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]
Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression and metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta1 (a) or beta4 (b) receptor (green) and FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/19/10/2912), licensed under a CC-BY license.Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]
Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]
Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining shown in green.Applications for FAK Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAK
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Documents for FAK Antibody
Product Specific Notices for FAK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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