Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 14C2

Concentration

1.0 mg/ml

View all Fas Ligand/TNFSF6 Antibodies »

Product Specifications

Immunogen

Made to Human CD178

Localization

Cell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface.

Specificity

NB100-63072 recognizes the human CD178 cell surface antigen, a 40kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis. Clone 14C2 is not reported to have a blocking function.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fas Ligand/TNFSF6 Antibody (14C2)

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) - BSA Free [NB100-63072]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) - BSA Free [NB100-63072]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) [NB100-63072] - Analysis using the PE conjugate of NB100-63072. Staining of permeabilised Jurkat cells.
Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) - BSA Free [NB100-63072]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) - BSA Free [NB100-63072]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (14C2) [NB100-63072] - Staining of permeabilised Jurkat cells with MOUSE ANTI HUMAN CD178
Fas Ligand/TNFSF6 Antibody (14C2) - BSA Free

Flow Cytometry: Mouse Monoclonal Fas Ligand/TNFSF6 Antibody (14C2) [NB100-63072] -

Flow Cytometry: Mouse Monoclonal Fas Ligand/TNFSF6 Antibody (14C2) [NB100-63072] - NK cells were magnetically sorted from resting PBMC after thawing and stained for CD178 (FasL) PE-Cy5.5. The left plot depicts isotype staining with PE-Cy5.5 Mouse IgG1. Analysis using PE/Cy5.5 conjugated version of Fas Ligand/TNFSF6 (NB100-63072PECY55). Image from a verified customer review.

Applications for Fas Ligand/TNFSF6 Antibody (14C2)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:25 - 1:50

Immunoprecipitation

1:10 - 1:500
Application Notes
Results maybe enhanced using membrane permeabilisation in Flow Cytometry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fas Ligand/TNFSF6

Fas / APO-1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand (Fas-L) or an anti-Fas cross-linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas-L system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand (Fas-L), which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas-L system. Fas is believed to be involved in various autoimmune diseases including, ulcerative colitis, Graves disease, and rheumatoid arthritis. Fas expression on gastric epithelial cells in patients infected with H.Pylori is responsible for the accelerated apoptosis of the cells. Serum Fas-L concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension.

Alternate Names

CD178, CD95L, FASLG, TNFSF6

Entrez Gene IDs

356 (Human)

Gene Symbol

FASLG

UniProt

P48023

Additional Fas Ligand/TNFSF6 Products

Product Documents for Fas Ligand/TNFSF6 Antibody (14C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fas Ligand/TNFSF6 Antibody (14C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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