Feline CXCR4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4287
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Feline
Cited:
Feline
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 374606
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with feline CXCR4
Met1-Ser353
Accession # P56498
Met1-Ser353
Accession # P56498
Specificity
Detects feline CXCR4. Stains feline CXCR4 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Feline CXCR4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Feline peripheral blood mononuclear cells
Sample: Feline peripheral blood mononuclear cells
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed feline peripheral blood mononuclear cells
Sample: Immersion fixed feline peripheral blood mononuclear cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR4
CXCR4, also known as fusin and CD184, is a 7TM G protein-linked chemokine receptor. CXCR4 is expressed on B cells, monocytes, CD4+ T cells, colonic epithelial cells, and astrocytes and binds SDF-1/CXCL12. CXCR4 is up‑regulated on mammary tumors. CXCR4, in cooperation with CD134 and heparan sulfate proteoglycans, functions as a co-receptor for feline immunodeficiency virus (FIV) entry into cells. Feline CXCR4 shares 97%, 95%, 88%, and 90% aa sequence identity with canine, human, mouse, and rat CXCR4, respectively.
Long Name
C-X-C Motif Chemokine Receptor 4
Alternate Names
CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS
Gene Symbol
CXCR4
UniProt
Additional CXCR4 Products
Product Documents for Feline CXCR4 Antibody
Product Specific Notices for Feline CXCR4 Antibody
For research use only
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