FGF-21 Antibody (OTI2F10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00645
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2F10
Concentration
1.4 mg/ml
Product Summary for FGF-21 Antibody (OTI2F10)
Immunogen
Human recombinant protein fragment corresponding to amino acids 29-209 of human FGF21(NP_061986) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for FGF-21 Antibody (OTI2F10)
Western Blot: FGF-21 Antibody (OTI2F10) [NBP2-00645]
Western Blot: FGF21 Antibody (2F10) [NBP2-00645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.Immunocytochemistry/ Immunofluorescence: FGF-21 Antibody (OTI2F10) [NBP2-00645]
Immunocytochemistry/Immunofluorescence: FGF-21 Antibody (2F10) [NBP2-00645] - Anti-FGF21 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF21(RC204538) at 1:100
Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] Staining of paraffin-embedded Human tonsil using anti-FGF21 mouse monoclonal antibody.
Applications for FGF-21 Antibody (OTI2F10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.4 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGF-21
Long Name
Fibroblast Growth Factor 21
Alternate Names
FGF21
Entrez Gene IDs
26291 (Human)
Gene Symbol
FGF21
Additional FGF-21 Products
Product Documents for FGF-21 Antibody (OTI2F10)
Product Specific Notices for FGF-21 Antibody (OTI2F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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