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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Dot Blot, ELISA, Immunohistochemistry, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FG9-77

Format

Azide and BSA Free

Concentration

2.0 mg/ml

Product Summary for FGF-9 Antibody (FG9-77) - Azide and BSA Free

Immunogen

Recombinant mouse FGF-9

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Secreted

Specificity

Monoclonal Anti-FGF9 reacts specifically with recombinant FGF9 (fibroblast growth factor 9). It does not cross-react with human FGF Acidic and FGF Basic.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF-9 Antibody (FG9-77) - Azide and BSA Free

Immunohistochemistry: FGF-9 Antibody (FG9-77) - Azide and BSA Free [NB600-623]

Immunohistochemistry: FGF-9 Antibody (FG9-77) - Azide and BSA Free [NB600-623]

Immunohistochemistry: FGF9 Antibody [NB600-623] - Photo courtesy of Andrew Siegel, TGEN. 1:1,000 dilution on cingulate gyrus brain tissue with no Parkinson's disease pathology.

Applications for FGF-9 Antibody (FG9-77) - Azide and BSA Free

Application
Recommended Usage

Dot Blot

5 ug/ml

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF-9

The protein encoded by the FGF9 gene is a member of the fibroblast growth factor (FGF) family. FGF family members possessbroad mitogenic and cell survival activities, and are involved in a variety of biological processes, includingembryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolatedas a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, thisprotein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homologof this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed amale-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. (provided by RefSeq)

Long Name

Fibroblast Growth Factor 9

Alternate Names

FGF9

Entrez Gene IDs

2254 (Human); 14180 (Mouse)

Gene Symbol

FGF9

UniProt

Additional FGF-9 Products

Product Documents for FGF-9 Antibody (FG9-77) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGF-9 Antibody (FG9-77) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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