FGFR3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1509
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide: KDLLPPAPPSSGGSRT, corresponding to amino acids 792-806 of the cytoplasmic region of Human FGFR3 with N-terminal added lysine.
Reactivity Notes
Cross-reacts with Human.Not yet tested in other species.
Localization
Plasma membrane; Single-pass type I membrane protein
Specificity
Does not react with human FGFR1 or FGFR2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FGFR3 Antibody
Western Blot: FGFR3 Antibody [NB600-1509]
Western Blot: FGFR3 Antibody [NB600-1509] - Western blot analysis of apoptotic markers following 24 and 48 hrs of selenite (5 uM), GS-Se-SG (5 uM) and Se-DL-cystine (100 uM) treatment. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1111/jcmm.12214), licensed under a CC-BY license.Western Blot: FGFR3 Antibody [NB600-1509] -
Western Blot: FGFR3 Antibody [NB600-1509] - (A) Western blot analysis of apoptotic markers following 24 & 48 hrs of selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) treatment. (B) Western blot analysis of autophagic & ER stress markers, evaluated after 1, 3, 6 & 18 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM). (C) Acridine orange staining of cells after 12 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) (magnification 40 ×). (D) High-resolution transmission electron microscopy micrographs of control & 24 hrs Se-DL-cystine-treated cells, showing the presence of autophagosome, as evident from double-layered membrane structure with inclusion bodies. (E) Western blot detection of ubiquitinylated proteins after treatments with different selenium compounds (similar doses as previous) for 6 hrs. Five microgram cell lysate was loaded into each lane. The lane intensity was analysed by using Adobe Photoshop program (version CS5). beta-actin was used as a loading control (Fig. 4A & B). C: Control; 1: selenite; 2: GS-Se-SG; 3: Se-DL-cystine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24400844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for FGFR3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:250
Immunohistochemistry-Paraffin
1:250
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGFR3
Long Name
Fibroblast Growth Factor Receptor 3
Alternate Names
CD333, CEK, FGF R3, JTK4
Entrez Gene IDs
2261 (Human)
Gene Symbol
FGFR3
Additional FGFR3 Products
Product Documents for FGFR3 Antibody
Product Specific Notices for FGFR3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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