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FGFR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1509

Novus Biologicals, part of Bio-Techne
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NB600-1509
NB600-1509-0.025ml

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Synthetic peptide: KDLLPPAPPSSGGSRT, corresponding to amino acids 792-806 of the cytoplasmic region of Human FGFR3 with N-terminal added lysine.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Plasma membrane; Single-pass type I membrane protein

Specificity

Does not react with human FGFR1 or FGFR2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR3 Antibody

Western Blot: FGFR3 Antibody [NB600-1509]

Western Blot: FGFR3 Antibody [NB600-1509]

Western Blot: FGFR3 Antibody [NB600-1509] - Western blot analysis of apoptotic markers following 24 and 48 hrs of selenite (5 uM), GS-Se-SG (5 uM) and Se-DL-cystine (100 uM) treatment. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1111/jcmm.12214), licensed under a CC-BY license.
FGFR3 Antibody

Western Blot: FGFR3 Antibody [NB600-1509] -

Western Blot: FGFR3 Antibody [NB600-1509] - (A) Western blot analysis of apoptotic markers following 24 & 48 hrs of selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) treatment. (B) Western blot analysis of autophagic & ER stress markers, evaluated after 1, 3, 6 & 18 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM). (C) Acridine orange staining of cells after 12 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) (magnification 40 ×). (D) High-resolution transmission electron microscopy micrographs of control & 24 hrs Se-DL-cystine-treated cells, showing the presence of autophagosome, as evident from double-layered membrane structure with inclusion bodies. (E) Western blot detection of ubiquitinylated proteins after treatments with different selenium compounds (similar doses as previous) for 6 hrs. Five microgram cell lysate was loaded into each lane. The lane intensity was analysed by using Adobe Photoshop program (version CS5). beta-actin was used as a loading control (Fig. 4A & B). C: Control; 1: selenite; 2: GS-Se-SG; 3: Se-DL-cystine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24400844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FGFR3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGFR3

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human)

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for FGFR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGFR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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