FKBP12 Antibody (OTI3B3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02093

Novus Biologicals, part of Bio-Techne
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NBP2-02093
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FKBP12 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FKBP1A (NP_000792) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

11.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FKBP12 Antibody (OTI3B3)

Western Blot: FKBP12 Antibody (OTI3B3) [NBP2-02093]

Western Blot: FKBP12 Antibody (OTI3B3) [NBP2-02093]

Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.
Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] Staining of paraffin-embedded Human Kidney tissue using anti-FKBP12 mouse monoclonal antibody.
Flow Cytometry: FKBP12 Antibody (OTI3B3) [NBP2-02093]

Flow Cytometry: FKBP12 Antibody (OTI3B3) [NBP2-02093]

Flow Cytometry: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FKBP12 antibody, and then analyzed by flow cytometry.
View 1 more images

Applications for FKBP12 Antibody (OTI3B3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FKBP12

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq].

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Entrez Gene IDs

2280 (Human)

Gene Symbol

FKBP1A

Additional FKBP12 Products

Product Documents for FKBP12 Antibody (OTI3B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FKBP12 Antibody (OTI3B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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