FKBP12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14017

Novus Biologicals, part of Bio-Techne
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NBP2-14017
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FKBP12 Antibodies »

Product Summary for FKBP12 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FKBP12 Antibody

Western Blot: FKBP12 Antibody [NBP2-14017]

Western Blot: FKBP12 Antibody [NBP2-14017]

Western Blot: FKBP12 Antibody [NBP2-14017] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Hep-G2
Immunohistochemistry-Paraffin: FKBP12 Antibody [NBP2-14017]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NBP2-14017]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NBP2-14017] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for FKBP12 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP12

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Gene Symbol

FKBP1A

Additional FKBP12 Products

Product Documents for FKBP12 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FKBP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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