Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FKBP12 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid peptide near the center of human FKBP12. The immunogen is located within the first 50 amino acids of FKBP12. Amino Acid Squence: MLEDGKKFDSSRDRNKPF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FKBP12 Antibody - BSA Free

Western Blot: FKBP12 AntibodyBSA Free [NBP2-41272]

Western Blot: FKBP12 AntibodyBSA Free [NBP2-41272]

Western Blot: FKBP12 Antibody [NBP2-41272] - Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 ug/ml.
FKBP12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] -

Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] - Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 u/mL.
FKBP12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] -

Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] - Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 ug/mL.

Applications for FKBP12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 -20 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP12

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Gene Symbol

FKBP1A

Additional FKBP12 Products

Product Documents for FKBP12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FKBP12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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