FKBP12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99149

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FKBP12 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human FKBP12 (Accession#: NP_463460; Met 1-Glu 108)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FKBP12 Antibody

Western Blot: FKBP12 Antibody [NBP2-99149]

Western Blot: FKBP12 Antibody [NBP2-99149]

Western Blot: FKBP12 Antibody [NBP2-99149] - Anti-FKBP12 rabbit polyclonal antibody at 1:500 dilution. Lane A: SH-SY5Y Whole Cell Lysate. Lane B: HL-60 Whole Cell Lysate. Lane C: U937 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 12 kDa (We are unsure as to the identity of these extra bands).

Applications for FKBP12 Antibody

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP12

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Gene Symbol

FKBP1A

Additional FKBP12 Products

Product Documents for FKBP12 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FKBP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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