Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).

Reactivity Notes

Cross reacts with Human and Mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FoxP3 Antibody (3G3) [FITC]

Flow Cytometry: FoxP3 Antibody (3G3) [FITC] [NBP2-26668] - FOXP3 Antibody (3G3) [FITC] [NBP2-26668] - Analysis of FOXP3 in mouse splenocytes stimulated with anti-mCD3 (5 ug/ml) and rmIL-2 (10 ng/ml) for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody , anti-FOXP3 FITC conjugate this antibody .
Flow Cytometry: FoxP3 Antibody (3G3) [FITC] [NBP2-26668] - FOXP3 Antibody (3G3) [FITC] [NBP2-26668] - Analysis of FOXP3 in human PBMCs stimulated with anti-hCD3 (5 ug/ml) and rhIL-2 (10 ng/ml) for 48 hours using isotype control (left) and this antibody. 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate , anti-CD25 APC conjugate , anti-FOXP3 FITC conjugate .

Applications for FoxP3 Antibody (3G3) [FITC]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FoxP3

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FOXP3, a trascription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FOXP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FOXP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (3G3) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FoxP3 Antibody (3G3) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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