Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G3

Format

Azide Free

Concentration

1.0 mg/ml

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FoxP3 Antibody (3G3) - Azide Free

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297]

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297]

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).
Western Blot: FoxP3 Antibody (3G3)Azide Free [NBP2-33297]

Western Blot: FoxP3 Antibody (3G3)Azide Free [NBP2-33297]

Western Blot: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.
Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297]

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297]

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.
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Applications for FoxP3 Antibody (3G3) - Azide Free

Application
Recommended Usage

Flow (Intracellular)

100 ng/2x10^6 cells

Western Blot

2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin. Does not contain BSA

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP3

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (3G3) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FoxP3 Antibody (3G3) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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