What is the immunogen sequence of this FoxP3 antibody?

Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FoxP3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]

Reactivity Notes

This antibody does not appear to detect the mouse FOXP3 protein by western blot, but does work on mouse tissue for IHC.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FoxP3 Antibody - BSA Free

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245]

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse stained with FoxP3 and CD4.

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245]

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245] - FoxP3 Antibody [NB600-245] - Healthy thymus from a C57BL/6 mouse incubated only with CD4 and no Foxp3.

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB600-245]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-245] - Fox P3 was detected in immersion fixed paraffin-embedded sections of human tonsil using rabbit anti-human antibody (Catalog # NB600-245) at 1:100 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent (Catalog # VC003) with DAB chromogen (brown) and counterstained with hematoxylin (blue).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Western Blot: FoxP3 AntibodyBSA Free [NB600-245]

Western Blot: FOXP3 Antibody [NB600-245] - Detection of human FOXP3 using NB 600-245. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector

Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD8A and FOXP3 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Dual RNAscope ISH-IHC: FoxP3 Antibody [NB600-245] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD4 and FOXP3 antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

FoxP3 in MCF7 Human Cell Line.

FoxP3 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-FoxP3 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-245AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

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Applications for FoxP3 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:1000

Immunohistochemistry-Frozen

1:50

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:1000-1:5000

Application Notes

This FOXP3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. By Western blot, this antibody recognizes bands at ~47.2 kDa representing FOXP3. For IHC-Fr it has been used in mouse thymus from an influenza-infected mouse or thymus from healthy mice (C57BL/6).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP3

FOXP3 (forkhead box protein P3) belongs to forkhead/winged-helix gene family of transcription factors that regulates the development and function of immune system. Localized in the nucleus, FOXP3 interacts with IKZF3 and is a crucial factor for the generation of CD4+CD25+ regulatory T cells (Tregs) and high levels expression of FOXP3 is essential for Treg's suppressive functions. SIRT1 facilitates FOXP3's decetylation, whereas, its acetylation on lysine residues causes its stabilization and promotes differentiation of T-cells into induced Tregs. Mutations of the FOXP3 gene lead to X-linked autoimmunity-allergic dysregulation syndrome in humans (also called immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome or IPEX) and the lymphoproliferative disease in Scurfy mouse. The FOXP3 X-linked recessive mutation results in lethality in hemizygous male mice soon after birth, and is characterized by excessive proliferation of CD4+ T cells, extensive multi-organ infiltration by leukocytes and systemic elevation of numerous cytokines. FoxP3 has also been shown to function as a tumor suppressor in several types of cancer.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for FoxP3 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download IHC-F Protocol

Download IHC-P Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for FoxP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FoxP3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-245

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for FoxP3 Antibody - BSA Free

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245]

Immunohistochemistry-Frozen: FoxP3 Antibody - BSA Free [NB600-245]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB600-245]

Western Blot: FoxP3 AntibodyBSA Free [NB600-245]

FoxP3 in MCF7 Human Cell Line.

Applications for FoxP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: FoxP3

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional FoxP3 Products

Product Documents for FoxP3 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for FoxP3 Antibody - BSA Free