FoxP3 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-245V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FoxP3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431). [UniProt# Q9BZS1]

Reactivity Notes

This antibody does not appear to detect the mouse FOXP3 protein by western blot, but does work on mouse tissue for IHC.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for FoxP3 Antibody [DyLight 405]

Application
Recommended Usage

Dual RNAscope ISH-IHC

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FoxP3

FOXP3 (forkhead box protein P3) belongs to forkhead/winged-helix gene family of transcription factors that regulates the development and function of immune system. Localized in the nucleus, FOXP3 interacts with IKZF3 and is a crucial factor for the generation of CD4+CD25+ regulatory T cells (Tregs) and high levels expression of FOXP3 is essential for Treg's suppressive functions. SIRT1 facilitates FOXP3' decetylation, whereas, its acetylation on lysine residues causes its stabilization and promotes differentiation of T-cells into induced Tregs. Mutations of the FOXP3 gene lead to X-linked autoimmunity-allergic dysregulation syndrome in humans (also called immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome or IPEX) and the lymphoproliferative disease in Scurfy mouse. The FOXP3 X-linked recessive mutation results in lethality in hemizygous male mice soon after birth, and is characterized by excessive proliferation of CD4+ T cells, extensive multi-organ infiltration by leukocytes and systemic elevation of numerous cytokines. FoxP3 has also been shown to function as a tumor suppressor in several types of cancer.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FoxP3 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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