Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Bovine (91%), Canine (91%), Equine (93%), Feline (90%), Goat (91%), Golden Syrian Hamster (91%), Guinea Pig (92%), Mouse (91%), Porcine (91%), Primate (96%), Rabbit (91%), Rat (91%), Sheep (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 6E8.2F5

Format

BSA Free

Concentration

1.0 mg/ml

View all FSHR Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the human FSH R protein sequence (between residues 1-200). [Swiss-Prot P23945]

Reactivity Notes

Immunogen's sequence similarity with other species: Chicken and Duck (70%), Xenopus (71%).

Localization

Cell membrane, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for FSHR Antibody (6E8.2F5) - BSA Free

Western Blot: FSHR Antibody (6E8.2F5)BSA Free [NBP2-36489]

Western Blot: FSHR Antibody (6E8.2F5)BSA Free [NBP2-36489]

Western Blot: FSH R Antibody (6E8.2F5) [NBP2-36489] - WB analysis of (A) 15kDa partial recombinant Human FSHR protein and (B) lysate of HepG2 cells using FSH R antibody (clone 6E8.2F5) at 3ug/ml concentration.
Immunohistochemistry-Paraffin: FSHR Antibody (6E8.2F5) - BSA Free [NBP2-36489]

Immunohistochemistry-Paraffin: FSHR Antibody (6E8.2F5) - BSA Free [NBP2-36489]

Immunohistochemistry-Paraffin: FSH R Antibody (6E8.2F5) [NBP2-36489] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal adenocarcinoma using FSH R antibody (clone 6E8.2F5) at 5 ug/ml concentration. The columnar cells depicted strong membranous staining with weaker cytoplasmic signal for FSHR protein.
Immunohistochemistry-Paraffin: FSHR Antibody (6E8.2F5) - BSA Free [NBP2-36489]

Immunohistochemistry-Paraffin: FSHR Antibody (6E8.2F5) - BSA Free [NBP2-36489]

Immunohistochemistry-Paraffin: FSH R Antibody (6E8.2F5) [NBP2-36489] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using FSH R antibody (clone 6E8.2F5) at 5 ug/ml concentration.
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Applications for FSHR Antibody (6E8.2F5) - BSA Free

Application
Recommended Usage

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

2-3 ug/ml
Application Notes
The canonical isoform of FSHR is 78.3 kDa, however, it undergoes processing via glycosylation and the glycosylated protein runs at higher than predicted molecular weight. Under non-reducing conditions, FSHR may form dimers via disulfide bond formation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FSHR

Follicle-stimulating hormone (FSH), also called follitropin, belongs to the family of glycoprotein hormones that also includes luteininizing hormone and thyroid-stimulating hormone. These hormones are secreted by the pituitary and exist as heterodimers, consisting of a common alpha subunit and a homologous but distinct beta subunit. While the alpha subunit of FSH is involved in the binding of FSH to the receptor, follicle-stimulating hormone receptor (FSHR), the beta subunit stabilizes this interaction. This heterodimer regulates a variety of processes, including secretion, post-translational modification and signal transduction. Both FSH and FSHR are localized to Sertoli cells.

Long Name

Follicle Stimulating Hormone Receptor

Alternate Names

FSH R, LGR1, ODG1

Entrez Gene IDs

2492 (Human); 14309 (Mouse); 25449 (Rat)

Gene Symbol

FSHR

UniProt

P23945 (Human)

Additional FSHR Products

Product Documents for FSHR Antibody (6E8.2F5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FSHR Antibody (6E8.2F5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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