GAD1/GAD67 Antibody (CL2914)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46638

Novus Biologicals, part of Bio-Techne
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NBP2-46638
NBP2-46638-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2914

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAD1/GAD67 Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence QSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRS

Epitope

TETDFSNLFA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAD1/GAD67 Antibody (CL2914)

Western Blot: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Western Blot: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Western Blot: GAD1/GAD67 Antibody (CL2914) [NBP2-46638] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (CL2914) [NBP2-46638] - Staining of human liver shows absence of immunoreactivity (negative control).
Immunohistochemistry: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2914) [NBP2-46638]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2914) [NBP2-46638] - Staining of rat cerebral cortex shows strong immunoreactivity in GABAergic synapses on pyramidal neurons.
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Applications for GAD1/GAD67 Antibody (CL2914)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human)

Gene Symbol

GAD1

UniProt

Q99259

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (CL2914)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD1/GAD67 Antibody (CL2914)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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