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GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43813

Novus Biologicals, part of Bio-Techne
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NBP3-43813

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2914

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q99259, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free

GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free Western Blot: GAD1/GAD67 Antibody [NBP3-43813]

Western Blot: GAD1/GAD67 Antibody [NBP3-43813]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43813]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43813]

Staining of human cerebellum shows strong immunoreactivity in Purkinje cells, as well as in fibers in granular and molecular layers.
GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43813]

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43813]

Staining of human liver shows absence of immunoreactivity (negative control).

Applications for GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAD1/GAD67 Antibody (CL2914) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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