GAD2/GAD65 Antibody (CL2736)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46629

Novus Biologicals, part of Bio-Techne
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NBP2-46629
NBP2-46629-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2736

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAD2/GAD65 Antibodies »

Product Summary for GAD2/GAD65 Antibody (CL2736)

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence SGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLP

Epitope

AACACDQKPC

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAD2/GAD65 Antibody (CL2736)

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.
Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.
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Applications for GAD2/GAD65 Antibody (CL2736)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Entrez Gene IDs

2572 (Human)

Gene Symbol

GAD2

UniProt

Q05329

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (CL2736)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAD2/GAD65 Antibody (CL2736)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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