GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43815

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2736

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GAD2/GAD65 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q05329, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free

GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP3-43815]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP3-43815]

Analysis in human cerebral cortex and tonsil tissues using NBP3-43815 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.
GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free Western Blot: GAD2/GAD65 Antibody [NBP3-43815]

Western Blot: GAD2/GAD65 Antibody [NBP3-43815]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP3-43815]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP3-43815]

Staining of human cerebellum shows moderate to strong positivity in GABAergic neuronal processes.
View 8 more images

Applications for GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GAD2/GAD65 Antibody (CL2736) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.