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Galectin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89791

Novus Biologicals, part of Bio-Techne
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NBP1-89791
NBP1-89791-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Galectin-1 Antibody

Western Blot: Galectin-1 Antibody [NBP1-89791]

Western Blot: Galectin-1 Antibody [NBP1-89791]

Western Blot: Galectin-1 Antibody [NBP1-89791] - Analysis in human cell lines PC-3 and Caco-2 using anti-LGALS1 antibody. Corresponding LGALS1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Western Blot: Galectin-1 Antibody [NBP1-89791]

Western Blot: Galectin-1 Antibody [NBP1-89791]

Western Blot: Galectin-1 Antibody [NBP1-89791] - Analysis using Anti-LGALS1 antibody NBP1-89791 (A) shows similar pattern to independent antibody NBP2-76490 (B).
Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP1-89791]

Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP1-89791]

Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP1-89791] - Staining of human liver shows moderate cytoplasmic positivity in a subset of hepatocytes.

Applications for Galectin-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-1

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Gene Symbol

LGALS1

Additional Galectin-1 Products

Product Documents for Galectin-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Galectin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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