Galectin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16581
![Western Blot: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0007.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581]")
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Galectin-1 Antibody
Immunogen
Full length human Galectin-1 Recombinant protein.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Canine (82%), Porcine (85%), Bovine (86%).
Localization
Secreted; extracellular space; extracellular matrix
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Galectin-1 Antibody
![Western Blot: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0014.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581]")
Western Blot: Galectin-1 Antibody [NBP2-16581]
Western Blot: Galectin-1 Antibody [NBP2-16581] - Wild-type (WT) and Galectin-1 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]")
Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]
Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody detects Galectin-1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin-1 protein stained by Galectin-1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.![Western Blot: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0006.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581]")
Western Blot: Galectin-1 Antibody [NBP2-16581]
Western Blot: Galectin-1 Antibody [NBP2-16581] - A: BCL-1.![Western Blot: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0010.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581]")
![Western Blot: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0012.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581]")
![Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]")
![Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]")
![Immunoprecipitation: Galectin-1 Antibody [NBP2-16581]](https://resources.bio-techne.com/images/products/Galectin-1-Antibody-Immunoprecipitation-NBP2-16581-img0009.jpg "Immunoprecipitation: Galectin-1 Antibody [NBP2-16581]")
Applications for Galectin-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Galectin-1
Additional Galectin-1 Products
Product Documents for Galectin-1 Antibody
Product Specific Notices for Galectin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.