GDNF Antibody (11D2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26556

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26556-100ul
NBP3-26556-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 11D2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GDNF Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human GDNF [UniProt P39905]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GDNF Antibody (11D2)

GDNF Antibody (11D2)

Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] -

Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] - Immunoprecipitating GDNF in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26556 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26556 (3ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (20ug).
GDNF Antibody (11D2)

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] -

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
GDNF Antibody (11D2)

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] -

Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Applications for GDNF Antibody (11D2)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: GDNF

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. ; FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. ; DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Gene Symbol

GDNF

Additional GDNF Products

Product Documents for GDNF Antibody (11D2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GDNF Antibody (11D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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