Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 20

Format

BSA Free

Concentration

1.0 mg/ml

View all Gelsolin/GSN Antibodies »

Product Specifications

Immunogen

Gelsolin purified from human plasma

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Gelsolin/GSN Antibody (20) - BSA Free

Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]

Western Blot: Gelsolin/GSN Antibody (20)BSA Free [NBP1-05161]

Western Blot: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]

Immunocytochemistry/ Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]

Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human cell line U-251 MG shows positivity in actin filaments. Antibody dilution: 1:2000
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (20) - BSA Free [NBP1-05161] - Human Kidney.

Applications for Gelsolin/GSN Antibody (20) - BSA Free

Application
Recommended Usage

ELISA

1:4000

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:6000

Immunohistochemistry-Paraffin

1:6000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05161 does not work as a capture antibody in sandwich ELISA. WB: NBP1-05161 can be used in Western blotting. IHC: NBP1-05161 can be used in Immunohistochemistry. IF: NBP1-05161 can be used in Immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Gelsolin/GSN

Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (20) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Gelsolin/GSN Antibody (20) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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