Geminin Antibody (GMNN/3665)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13992

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # GMNN/3665

Concentration

0.2 mg/ml

View all Geminin Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Geminin protein (exact sequence is proprietary) (Uniprot: O75496)

Localization

Nuclear expression in highly proliferating cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14128)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Geminin Antibody (GMNN/3665)

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunocytochemistry/Immunofluorescence: Geminin Antibody (GMNN/3665) [NBP3-13992] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using Geminin Antibody (GMNN/3665) followed by goat anti-mouse IgG-CF488 (green).
Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992] - Formalin-fixed, paraffin-embedded human spleen stained with Geminin antibody (GMNN/3665). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992]

Immunohistochemistry-Paraffin: Geminin Antibody (GMNN/3665) [NBP3-13992] - Formalin-fixed, paraffin-embedded human testis stained with Geminin antibody (GMNN/3665). Inset: PBS instead of primary antibody; secondary only negative control.
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Applications for Geminin Antibody (GMNN/3665)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

UniProt

O75496 (Human)

Additional Geminin Products

Product Documents for Geminin Antibody (GMNN/3665)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Geminin Antibody (GMNN/3665)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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