Geminin Antibody (GMNN/3665) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14128
![Protein Array: Geminin Antibody (GMNN/3665) - Azide and BSA Free [NBP3-14128]](https://resources.bio-techne.com/images/products/Geminin-Antibody-GMNN-3665-Azide-and-BSA-Free-Protein-Array-NBP3-14128-img0001.jpg "Protein Array: Geminin Antibody (GMNN/3665) - Azide and BSA Free [NBP3-14128]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # GMNN/3665
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human Geminin protein (exact sequence is proprietary) (Uniprot: O75496)
Localization
Nuclear expression in highly proliferating cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13992).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Geminin Antibody (GMNN/3665) - Azide and BSA Free
Protein Array: Geminin Antibody (GMNN/3665) - Azide and BSA Free [NBP3-14128]
Protein Array: Geminin Antibody (GMNN/3665) - Azide and BSA Free [NBP3-14128] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Geminin Antibody (GMNN/3665).
Geminin Antibody (GMNN/3665)
Formalin-fixed, paraffin-embedded human spleen stained with Geminin antibody (GMNN/3665). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Geminin Antibody (GMNN/3665) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Geminin
Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.
Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.
Alternate Names
Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3
Gene Symbol
GMNN
Additional Geminin Products
Product Documents for Geminin Antibody (GMNN/3665) - Azide and BSA Free
Product Specific Notices for Geminin Antibody (GMNN/3665) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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