Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Girdin Antibodies »

Product Summary for Girdin Antibody

Immunogen

The immunogen for this product maps to a region between residue 1425 and 1475 of human Coiled-Coil Domain Containing 88A using the numbering given in entry NP_001129069.1 (GeneID 55704).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Girdin Antibody

Western Blot: Girdin Antibody [NBP1-71857]

Western Blot: Girdin Antibody [NBP1-71857]

Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Girdin Antibody [NBP1-71857]

Western Blot: Girdin Antibody [NBP1-71857]

Western Blot: Girdin Antibody [NBP1-71857] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71856 , which recognizes an upstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.

Applications for Girdin Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Girdin

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Long Name

Girders of Actin Filament

Alternate Names

APE, CCDC88A, GIV, GRDN, HkRP1

Gene Symbol

CCDC88A

UniProt

NP_001129069.1 (Human)

Additional Girdin Products

Product Documents for Girdin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Girdin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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