GLI-1 Antibody (OTI4E2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45872
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E2
Concentration
1 mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 755-1106 of human GLI1 (NP_005260) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
117.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GLI-1 Antibody (OTI4E2)
Western Blot: GLI-1 Antibody (OTI4E2) [NBP2-45872]
Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.Immunohistochemistry: GLI-1 Antibody (OTI4E2) [NBP2-45872]
Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Immunohistochemistry: GLI-1 Antibody (OTI4E2) [NBP2-45872]
Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for GLI-1 Antibody (OTI4E2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID:31611993)
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Knockdown Validated
Reproted in (PMID:31611993)
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GLI-1
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
Additional GLI-1 Products
Product Documents for GLI-1 Antibody (OTI4E2)
Product Specific Notices for GLI-1 Antibody (OTI4E2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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