GLI-1 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78259F

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-78259AF350

Alexa Fluor 405

NBP1-78259AF405

Alexa Fluor 488

NBP1-78259AF488

Alexa Fluor 532

NBP1-78259AF532

Alexa Fluor 594

NBP1-78259AF594

Alexa Fluor 647

NBP1-78259AF647

Alexa Fluor 700

NBP1-78259AF700

Alexa Fluor 750

NBP1-78259AF750

Allophycocyanin

NBP1-78259APC

Biotin

NBP1-78259B

CoraFluor 1

NBP1-78259CL1

DyLight 350

NBP1-78259UV

DyLight 405

NBP1-78259V

DyLight 488

NBP1-78259G

DyLight 550

NBP1-78259R

DyLight 594

NBP1-78259DL594

DyLight 650

NBP1-78259C

DyLight 680

NBP1-78259FR

DyLight 755

NBP1-78259IR

HRP

NBP1-78259H

Janelia Fluor 525

NBP1-78259JF525

Janelia Fluor 549

NBP1-78259JF549

Janelia Fluor 585

NBP1-78259JF585

Janelia Fluor 635

NBP1-78259JF635

Janelia Fluor 646

NBP1-78259JF646

Janelia Fluor 669

NBP1-78259JF669

mFluor Violet 450 SE

NBP1-78259MFV450

mFluor Violet 500 SE

NBP1-78259MFV500

mFluor Violet 610 SE

NBP1-78259MFV610

PE

NBP1-78259PE

PerCP

NBP1-78259PCP

Forumulation

Catalog #

BSA Free

NBP1-78259

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GLI-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human Gli1 protein (between residues 150-200) [UniProt P08151]

Localization

Cytoplasm. Nucleus. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for GLI-1 Antibody [FITC]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

In-situ Hybridization

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GLI-1

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Gene Symbol

GLI1

Additional GLI-1 Products

Product Documents for GLI-1 Antibody [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for GLI-1 Antibody [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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