GloLIVE Human/Mouse SSEA-1 NL557 Live Cell Antibody

To detect SSEA-1 expression in live cells by immunocytochemical staining.

Why confirm marker expression in live cells before colony selection?

Maintaining pluripotent human embryonic stem cells and deriving induced pluripotent stem cells require colony selection and cell expansion. Picking colonies that contain pluripotent stem cells is typically achieved by manually analyzing colony morphology. Unfortunately, this critical process is time-consuming and labor-intensive.

In addition, morphology-based colony selection does not consistently guarantee pluripotent starting populations, since cells can fail to be fully reprogrammed or can naturally differentiate within a colony.

To address this need, R&D Systems offers GloLIVE™ antibodies that allow researchers to confirm stem cell marker expression in live cells before colony selection.

Live pluripotent stem cell imaging:

• Promotes the selection of high quality, undifferentiated stem cell colonies to reduce experimental variation.
• Verifies stem cell pluripotency in 30 minutes, ensuring efficient use of time and reagents.
• Allows for the continued culture of pluripotent cells with no adverse effects on cell proliferation or stemness following staining.

• GloLIVE NL557-conjugated Mouse Anti-Human/Mouse SSEA-1 Monoclonal Antibody.
• Supplied as 50X concentration of antibody in 0.5 mL PBS

Azide free and endotoxin tested (= 5 EU/mL), this antibody can be used for 12 tests, if a staining volume of 2 mL is used.

 

Data

Detection of SSEA-1 in Live D3 Mouse Embryonic Stem Cells. The stem cell marker SSEA-1 was visualized in live D3 mouse embryonic stem cells, grown on irradiated mouse embryonic fibroblasts (Catalog # PSC001), using a GloLIVE NL557-conjugated Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # NLLC2155R; red). The nuclei were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-1 in mouse stem cells is an indicator of pluripotency.

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).