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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200). [UniProt P43220]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26398375).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLP-1R Antibody - BSA Free

Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.
Immunocytochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunocytochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.
Western Blot: GLP-1R AntibodyBSA Free [NLS1205]

Western Blot: GLP-1R AntibodyBSA Free [NLS1205]

Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.

Applications for GLP-1R Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 32232832)

Immunohistochemistry

10 - 20 ug/mL

Immunohistochemistry-Paraffin

10 - 20 ug/mL

Microarray

reported by customer review

Simple Western

1:50

Western Blot

1 - 2 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NLS1205 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GLP-1R

GLP1R, a Glucagon Receptor, has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. Glucagon-like peptide 1 is an incretin hormone produced by enteroendocrine L-cells in the intestinal mucosa. The hormone is released in response to food intake and plays an important role in maintaining blood glucose homeostasis. Stimulation of the GLP-1R by endogenous hormone induces multiple complementary mechanisms, which together result in a lowering of circulating blood glucose levels. These mechanisms include receptor-mediated enhancement of glucose-induced insulin secretion from pancreatic -cells, inhibition of gastric emptying with a delay in the gastrointestinal resorption of nutrients, inhibition of glucagon secretion, and inhibition of food intake. Desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM). GLP1R knockout mice are viable, develop normally, but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. Recently it has been shown that overexpression of glucagon-like peptide-1 receptor improves learning in rats (During et al. 2003). GLP1R has been reported in pancreas, brain, heart, kidney, lung, and stomach. ESTs have been isolated from kidney and skin libraries.

Long Name

Glucagon-like Peptide 1 Receptor

Alternate Names

GLP1R

Entrez Gene IDs

2740 (Human)

Gene Symbol

GLP1R

Additional GLP-1R Products

Product Documents for GLP-1R Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLP-1R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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