Glyoxalase I Antibody (3G6Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16375

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16375-100ul
NBP3-16375-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3G6Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glyoxalase I Antibodies »

Product Summary for Glyoxalase I Antibody (3G6Q1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Glyoxalase I (Q04760). MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glyoxalase I Antibody (3G6Q1)

Western Blot: Glyoxalase I Antibody (3G6Q1) [NBP3-16375]

Western Blot: Glyoxalase I Antibody (3G6Q1) [NBP3-16375]

Western Blot: Glyoxalase I Antibody (3G6Q1) [NBP3-16375] - Western blot analysis of extracts of various cell lines, using Glyoxalase I Rabbit mAb (NBP3-16375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for Glyoxalase I Antibody (3G6Q1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (3G6Q1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glyoxalase I Antibody (3G6Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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