Glyoxalase I Antibody (9F11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59369

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9F11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glyoxalase I Antibodies »

Product Summary for Glyoxalase I Antibody (9F11)

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Glyoxalase I Antibody (9F11)

Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369]

Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369]

Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Western Blot analysis of Glyoxalase I-BSA Conjugate showing detection of 67 kDa Glyoxalase I protein using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Lane 1: Molecular Weight Ladder (MW). Lane 2: Glyoxalase I-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (9F11) [NBP2-59369]

Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (9F11) [NBP2-59369]

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (9F11) [NBP2-59369] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369]

Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369]

Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369] - Flow Cytometry analysis using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.

Applications for Glyoxalase I Antibody (9F11)

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Glyoxalase I

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (9F11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glyoxalase I Antibody (9F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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