Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08555

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CPTC-GLO1-1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Glyoxalase I Antibodies »

Product Summary for Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

Immunogen

Recombinant full-length human Glyoxalase I protein (Uniprot: Q04760)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07311).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1)Azide and BSA Free [NBP3-08555]

Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1)Azide and BSA Free [NBP3-08555]

Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-08555] - Western Blot Analysis of HeLa cell lysate using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free [NBP3-08555]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free [NBP3-08555]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-08555] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free [NBP3-08555]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free [NBP3-08555]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-08555] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).
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Applications for Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glyoxalase I Antibody (CPTC-GLO1-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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