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Glyoxalase I Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30500

Novus Biologicals, part of Bio-Techne
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NBP3-30500

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 134 and 184

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glyoxalase I Antibody - BSA Free

Glyoxalase I Antibody

Immunoprecipitation:Glyoxalase I AntibodyNBP3-30500] -

Immunoprecipitation:Glyoxalase I AntibodyNBP3-30500] -Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase I antibody NBP3-30500 (lot NBP3-30500-1) used for IP at 6 µg per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I.. For blotting immunoprecipitated GLO1/Glyoxalase I, NBP3-30500 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for Glyoxalase I Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Glyoxalase I

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glyoxalase I Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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