GRK1 Antibody (D11) [Janelia Fluor® 585]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2776JF585

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB120-2776AF350

Alexa Fluor 405

NB120-2776AF405

Alexa Fluor 488

NB120-2776AF488

Alexa Fluor 532

NB120-2776AF532

Alexa Fluor 594

NB120-2776AF594

Alexa Fluor 647

NB120-2776AF647

Alexa Fluor 700

NB120-2776AF700

Alexa Fluor 750

NB120-2776AF750

Biotin

NB120-2776B

CoraFluor 1

NB120-2776CL1

DyLight 350

NB120-2776UV

DyLight 405

NB120-2776V

DyLight 488

NB120-2776G

DyLight 550

NB120-2776R

DyLight 594

NB120-2776DL594

DyLight 650

NB120-2776C

DyLight 680

NB120-2776FR

DyLight 755

NB120-2776IR

FITC

NB120-2776F

HRP

NB120-2776H

Janelia Fluor 525

NB120-2776JF525

Janelia Fluor 549

NB120-2776JF549

Janelia Fluor 635

NB120-2776JF635

Janelia Fluor 646

NB120-2776JF646

Janelia Fluor 669

NB120-2776JF669

mFluor Violet 450 SE

NB120-2776MFV450

mFluor Violet 500 SE

NB120-2776MFV500

mFluor Violet 610 SE

NB120-2776MFV610

Forumulation

Catalog #

BSA Free

NB120-2776

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Janelia Fluor 585

Antibody Source

Monoclonal Mouse IgG₁ Clone # D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRK1 Antibodies »

Product Specifications

Immunogen

Full length human GRK1.

Epitope

This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Specificity

Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This does not cross-react with other G protein-coupled kinases.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for GRK1 Antibody (D11) [Janelia Fluor® 585]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GRK1

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Long Name

G protein-coupled Receptor Kinase 1

Alternate Names

GPRK1, RHOK

Gene Symbol

GRK1

Additional GRK1 Products

Product Documents for GRK1 Antibody (D11) [Janelia Fluor® 585]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for GRK1 Antibody (D11) [Janelia Fluor® 585]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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