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GSK-3 alpha Antibody (9D5G1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-87048

Novus Biologicals, part of Bio-Techne
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NB110-87048
NB110-87048-0.025ml

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9D5G1

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for GSK-3 alpha Antibody (9D5G1) - BSA Free

Immunogen

Purified recombinant fragment of GSK-3 alpha expressed in E. Coli.

Specificity

GSK3 alpha (9D5G1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GSK-3 alpha Antibody (9D5G1) - BSA Free

Western Blot: GSK-3 alpha Antibody (9D5G1)BSA Free [NB110-87048]

Western Blot: GSK-3 alpha Antibody (9D5G1)BSA Free [NB110-87048]

Western Blot: GSK-3 alpha Antibody (9D5G1) [NB110-87048] - Analysis using GSK3 alpha mouse mAb against Hela (1) and PC-3 cell lysate.
Immunocytochemistry/ Immunofluorescence: GSK-3 alpha Antibody (9D5G1) - BSA Free [NB110-87048]

Immunocytochemistry/ Immunofluorescence: GSK-3 alpha Antibody (9D5G1) - BSA Free [NB110-87048]

Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody (9D5G1) [NB110-87048] - Analysis of Hela cells using GSK3 alpha mouse mAb showing cytoplasmic localization.

Applications for GSK-3 alpha Antibody (9D5G1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is useful for: Western Blot: 1:500- 1: 2,000 IF: 1:200- 1:1,000 ELISA: Propose dilution 1: 10,000.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 alpha

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Entrez Gene IDs

2931 (Human)

Gene Symbol

GSK3A

Additional GSK-3 alpha Products

Product Documents for GSK-3 alpha Antibody (9D5G1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSK-3 alpha Antibody (9D5G1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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