GSTM2 Antibody (006)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90040

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 006

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for GSTM2 Antibody (006)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human GSTM2 (Uniprot#: P28161; Met1-Lys218).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GSTM2 Antibody (006)

Western Blot: GSTM2 Antibody (006) [NBP2-90040]

Western Blot: GSTM2 Antibody (006) [NBP2-90040]

Western Blot: GSTM2 Antibody (006) [NBP2-90040] - Lane A: 293T Whole Cell LysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:28 kDa
Immunocytochemistry/ Immunofluorescence: GSTM2 Antibody (006) [NBP2-90040]

Immunocytochemistry/ Immunofluorescence: GSTM2 Antibody (006) [NBP2-90040]

Immunocytochemistry/Immunofluorescence: GSTM2 Antibody (006) [NBP2-90040] - Staining of GSTM2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human GSTM2 monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: GSTM2 Antibody (006) [NBP2-90040]

Immunoprecipitation: GSTM2 Antibody (006) [NBP2-90040]

Immunoprecipitation: GSTM2 Antibody (006) [NBP2-90040] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-GSTM2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GSTM2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa

Applications for GSTM2 Antibody (006)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSTM2

The GSTM2 gene codes for a glutathione S-transferase Mu 2 protein that functions as a catalyst in various reactions in eukaryotes and prokaryotes such as converting reduced gluthathione to a large number of exogenous and endogenous hydrophobic electrophiles. Two isoforms exist: isoform 1 is 218 amino acids long at 25 kDA while isoform 2 is 191 amino acids long at 22 kDA. GSTM2 participates in glutathione methabolism and the metapathway biotransformation and interacts with genes GSTM3, GSTM1, GSTA2, ADH1A, and ITECPR1. GSTM2 has been investigated in its role in carcinoma, hepatitis, arthritis, lung cancer, breast cancer susceptibility, rheumatoid arthritis, and diabetes mellitus.

Alternate Names

glutathione S-transferase mu 2 (muscle), MGC117303, muscle, S-(hydroxyalkyl)glutathione lyase M2

Gene Symbol

GSTM2

Additional GSTM2 Products

Product Documents for GSTM2 Antibody (006)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSTM2 Antibody (006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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