GSTM2 Antibody (CPTC-GSTMu2-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07346

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-GSTMu2-2

Concentration

0.2 mg/ml

View all GSTM2 Antibodies »

Product Summary for GSTM2 Antibody (CPTC-GSTMu2-2)

Immunogen

Recombinant human full-length GSTM2 protein (Uniprot: P28161)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08255)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for GSTM2 Antibody (CPTC-GSTMu2-2)

Western Blot: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Western Blot: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Western Blot: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346] - Western Blot Analysis of liver tissue lysate using GSTM2 Mouse Monoclonal Antibody (CPTC-GSTMu2-2).
Immunocytochemistry/ Immunofluorescence: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Immunocytochemistry/ Immunofluorescence: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Immunocytochemistry/Immunofluorescence: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling GSTM2 with GSTM2 Mouse Monoclonal Antibody (CPTC-GSTM2-2) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Immunohistochemistry-Paraffin: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Immunohistochemistry-Paraffin: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346]

Immunohistochemistry-Paraffin: GSTM2 Antibody (CPTC-GSTMu2-2) [NBP3-07346] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GSTM2 Mouse Monoclonal Antibody (CPTC-GSTM2-2).
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Applications for GSTM2 Antibody (CPTC-GSTMu2-2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GSTM2

The GSTM2 gene codes for a glutathione S-transferase Mu 2 protein that functions as a catalyst in various reactions in eukaryotes and prokaryotes such as converting reduced gluthathione to a large number of exogenous and endogenous hydrophobic electrophiles. Two isoforms exist: isoform 1 is 218 amino acids long at 25 kDA while isoform 2 is 191 amino acids long at 22 kDA. GSTM2 participates in glutathione methabolism and the metapathway biotransformation and interacts with genes GSTM3, GSTM1, GSTA2, ADH1A, and ITECPR1. GSTM2 has been investigated in its role in carcinoma, hepatitis, arthritis, lung cancer, breast cancer susceptibility, rheumatoid arthritis, and diabetes mellitus.

Alternate Names

glutathione S-transferase mu 2 (muscle), MGC117303, muscle, S-(hydroxyalkyl)glutathione lyase M2

Gene Symbol

GSTM2

UniProt

P28161 (Human)

Additional GSTM2 Products

Product Documents for GSTM2 Antibody (CPTC-GSTMu2-2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GSTM2 Antibody (CPTC-GSTMu2-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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