HA Tag Antibody (RM305)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61477
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM305
Concentration
1 mg/ml
Product Summary for HA Tag Antibody (RM305)
Immunogen
This HA Tag Antibody (RM305) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106).
Specificity
This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HA Tag Antibody (RM305)
Western Blot: HA Tag Antibody (RM305) [NBP2-61477] -
Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml
Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] -
Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] - Staining of LNCaP cells overexpressing HA-tag Bag1 protein, using NBP2-61477 at 0.01 ug/mlApplications for HA Tag Antibody (RM305)
Application
Recommended Usage
Flow Cytometry
0.1 - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
0.1 - 1 ug/mL
Immunohistochemistry
0.01 - 0.5 ug/ml
Immunoprecipitation
0.5 ug/ml - 2 ug/ml
Western Blot
0.1 ug/ml - 1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (RM305)
Product Specific Notices for HA Tag Antibody (RM305)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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