HA Tag Antibody (RM305) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26049
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM305
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This HA Tag Antibody (RM305) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106).
Specificity
This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HA Tag Antibody (RM305) - Azide and BSA Free
Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) - Azide and BSA Free [NBP3-26049] -
Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) - Azide and BSA Free [NBP3-26049] - Immunohistochemical staining of formalin fixed and paraffin embedded LNCaP cells overexpressing HA-tag Bag1 protein, using Clone RM305 at 0.01 ug/mL.
Western Blot: HA Tag Antibody (RM305) - Azide and BSA Free [NBP3-26049] -
Western Blot: HA Tag Antibody (RM305) - Azide and BSA Free [NBP3-26049] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using Clone RM305 at 0.1 ug/mL.Applications for HA Tag Antibody (RM305) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.1 - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
0.1 - 1 ug/mL
Immunohistochemistry
0.01 - 0.5 ug/ml
Immunoprecipitation
0.5 ug/ml - 2 ug/ml
Western Blot
0.1 ug/ml - 1 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (RM305) - Azide and BSA Free
Product Specific Notices for HA Tag Antibody (RM305) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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