HA Tag Horseradish Peroxidase-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # HAM0601

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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HAM0601
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA (detection), Western Blot

Label

HRP

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1049F

View all HA Tag Antibodies »

Product Specifications

Immunogen

HA Tag Peptide
Accession # ABB51961

Specificity

Detects N-terminal and C-terminal HA-tagged proteins in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA Tag Horseradish Peroxidase-conjugated Antibody

Detection of HA-tagged Proteins antibody by Western Blot.

Detection of HA-tagged Proteins by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-HA Tag Peptide HRP-conjugated Monoclonal Antibody (Catalog # HAM0601). Specific bands were detected for HA-tagged proteins at approximately 70 kDa and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for HA Tag Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Western Blot

1:1000-1:3000 dilution
Sample: HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a solution in PBS containing a preservative.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Entrez Gene IDs

23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)

Gene Symbol

HA

UniProt

ABB51961

Additional HA Tag Products

Product Documents for HA Tag Horseradish Peroxidase-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HA Tag Horseradish Peroxidase-conjugated Antibody

For research use only

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