HA Tag MAb (Clone 1049F)

R&D Systems, part of Bio-Techne | Catalog # MAB0601

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB0601
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Immunocytochemistry, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 1049F

View all HA Tag Antibodies »

Product Specifications

Immunogen

HA Tag peptide
Accession # ABB51961

Specificity

Detects N-terminal and C-terminal HA-tagged proteins in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA Tag MAb (Clone 1049F)

Detection of HA-tagged proteins antibody by Western Blot.

Detection of HA-tagged proteins by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, C-terminal HA-tagged TRANCE, and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HA-tagged proteins at approximately 70 kDa, 40 kDa, and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HA Tag antibody in NS0 Mouse Cell Line by Immunocytochemistry (ICC).

HA Tag in NS0 Mouse Cell Line.

HA was detected in immersion fixed NS0 mouse myeloma cell line transfected with HA-tagged WISP-2 using Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of HA-tagged proteins antibody by Simple WesternTM.

Detection of HA-tagged proteins by Simple WesternTM.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, loaded at 0.1 mg/mL. Specific bands were detected for HA-tagged proteins at approximately 87 and 106 kDa (as indicated) using 1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for HA Tag MAb (Clone 1049F)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with Hemagglutinin/HA-tagged WISP-2

Immunoprecipitation

1-4 µg/100 µg cell lysate
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, see our available Western blot detection antibodies.

Simple Western

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA

Western Blot

0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, C-terminal HA-tagged TRANCE, and C-terminal HA-tagged LRRN5

Reviewed Applications

Read 1 review rated 4 using MAB0601 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Entrez Gene IDs

23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)

Gene Symbol

HA

UniProt

ABB51961

Additional HA Tag Products

Product Documents for HA Tag MAb (Clone 1049F)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HA Tag MAb (Clone 1049F)

For research use only

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