Haptoglobin Antibody (OTI4H5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03008

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4H5

Concentration

1 mg/ml

View all Haptoglobin Antibodies »

Product Summary for Haptoglobin Antibody (OTI4H5)

Immunogen

Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Haptoglobin Antibody (OTI4H5)

Western Blot: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Western Blot: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Western Blot: Haptoglobin Antibody (OTI4H5) [NBP2-03008] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Haptoglobin.
Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (OTI4H5) [NBP2-03008] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.
Flow Cytometry: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Flow Cytometry: Haptoglobin Antibody (OTI4H5) [NBP2-03008]

Flow Cytometry: Haptoglobin Antibody (OTI4H5) [NBP2-03008] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Haptoglobin antibody, and then analyzed by flow cytometry.
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Applications for Haptoglobin Antibody (OTI4H5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Haptoglobin

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

HP, HP-1, hp2-alpha, HPA1S

Entrez Gene IDs

3240 (Human)

Gene Symbol

HP

Additional Haptoglobin Products

Product Documents for Haptoglobin Antibody (OTI4H5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Haptoglobin Antibody (OTI4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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