Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70925

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4H5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Haptoglobin Antibodies »

Product Summary for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

Western Blot: Haptoglobin Antibody (OTI4H5)Azide and BSA Free [NBP2-70925]

Western Blot: Haptoglobin Antibody (OTI4H5)Azide and BSA Free [NBP2-70925]

Western Blot: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Haptoglobi
Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925]

Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925]

Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.
Flow Cytometry: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925]

Flow Cytometry: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925]

Flow Cytometry: Haptoglobin Antibody (OTI4H5) - Azide and BSA Free [NBP2-70925] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Haptoglobin antibody, and then analyzed by flow cytometry.
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Applications for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Haptoglobin

Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.

Alternate Names

HP, HP-1, hp2-alpha, HPA1S

Gene Symbol

HP

Additional Haptoglobin Products

Product Documents for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Haptoglobin Antibody (OTI4H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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