Western blot shows the detection of recombinant HCoV-OC43-N. PVDF membrane was probed with 1 µg/mL of Mouse Anti-HCoV-OC43 Nucleocapsid Monoclonal Antibody (Catalog # MAB11289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nucleocapsid at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Nucleocapsid in Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line.

Nucleocapsid was detected in immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-HCoV-OC43 Nucleocapsid Monoclonal Antibody (Catalog # MAB11289) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Nucleocapsid in HEK293 cells transfected with HCOV-OC43-N or HEK293 cells transfected with an irrelevant protein cells by Flow Cytometry.

HEK293 cells transfected with HCOV-OC43-N or HEK293 cells transfected with an irrelevant protein were stained with Mouse Anti-HCoV-OC43 Nucleocapsid Monoclonal Antibody (Catalog # MAB11289, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for HCoV-OC43 Nucleocapsid Antibody

Application

Recommended Usage

Flow Cytometry

0.5 µg/10⁶ cells
Sample: HEK293 cells transfected with HCOV-OC43-N or HEK293 cells transfected with an irrelevant protein

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Transfected & Wild Type HEK293 Human Embryonic Kidney Cell Line

Western Blot

1 µg/mL
Sample: HCoV-OC43-N

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV-OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).

References

St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
Hurst, K.R. et al. (2009) J. Virol. 83:7221.
Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
Mourez, T. et al. (2007) J. Virol Methods. 139:175.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

UniProt

YP_009555245.1

Additional Nucleocapsid Products

Product Documents for HCoV-OC43 Nucleocapsid Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for HCoV-OC43 Nucleocapsid Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

HCoV-OC43 Nucleocapsid Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11289

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Nucleocapsid Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for HCoV-OC43 Nucleocapsid Antibody

Detection of HCoV-OC43 Nucleocapsid by Western Blot.