Histone H2AX Antibody (RM214)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18534

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-18534
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Key Product Details

Species Reactivity

Validated:

Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM214

Concentration

1 mg/ml

View all Histone H2AX Antibodies »

Product Summary for Histone H2AX Antibody (RM214)

Immunogen

A peptide corresponding to the C-terminus of human Histone H2AX

Specificity

This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX Antibody (RM214)

Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534]

Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534]

Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) [NBP3-18534]

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) [NBP3-18534]

Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (RM214) [NBP3-18534] - Immunocytochemistry of HeLa cells, using NBP3-18534 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H2AX Antibody (RM214)

Application
Recommended Usage

ELISA

0.2 ug/ml - 1 ug/ml

Immunocytochemistry/ Immunofluorescence

1 ug/mL - 2 ug/ml

Multiplex Immunoassay

0.2 ug/ml - 1 ug/ml

Western Blot

0.5 ug/mL - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (RM214)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX Antibody (RM214)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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