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Histone H2AX Antibody (RM214) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26044

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM214

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A peptide corresponding to the C-terminus of human Histone H2AX

Specificity

This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX Antibody (RM214) - Azide and BSA Free

Histone H2AX Antibody (RM214) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] -

Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Histone H2AX Antibody (RM214) - Azide and BSA Free

Western Blot: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] -

Western Blot: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] - Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.

Applications for Histone H2AX Antibody (RM214) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.2 ug/ml - 1 ug/ml

Immunocytochemistry/ Immunofluorescence

1 ug/mL - 2 ug/ml

Multiplex Immunoassay

0.2 ug/ml - 1 ug/ml

Western Blot

0.5 ug/mL - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (RM214) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H2AX Antibody (RM214) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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